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How to Use GraphPad Prism 5 for Data Analysis and Graphing
GraphPad Prism 5 is a powerful software for scientific data analysis and graphing. It can help you organize, analyze, and present your data in a clear and elegant way. Whether you need to perform basic or advanced statistical tests, fit nonlinear curves, create categorical or survival plots, or generate publication-quality graphs, Prism 5 can do it all.
In this article, we will show you how to use some of the main features of Prism 5, such as:
Entering and organizing your data
Choosing and running the appropriate analysis
Interpreting and reporting your results
Customizing and exporting your graphs
We will use some example data sets from the Prism User Guides and the Graph Portfolio to illustrate each step. You can download these data sets from the GraphPad website[^1^] and follow along.
Entering and Organizing Your Data
Prism 5 is designed to make data entry easy and intuitive. You can enter your data directly into the data tables, copy and paste from other programs, or import from text files. Prism 5 has different types of data tables for different types of analyses, such as:
XY tables for scatter plots, line graphs, and nonlinear regression
Column tables for bar graphs, box plots, t tests, ANOVA, and linear regression
Grouped tables for grouped bar graphs, stacked bar graphs, and two-way ANOVA
Contingency tables for chi-square tests and Fisher's exact test
Survival tables for Kaplan-Meier plots and log-rank tests
Parts-of-whole tables for pie charts and stacked area graphs
You can choose the type of table you need from the New Data Table dialog box when you create a new data set. You can also change the type of table later by clicking Change on the toolbar.
You can organize your data sets into different folders within a single project file. Each folder can contain multiple data sets, analyses, and graphs. You can rename, move, copy, or delete folders as needed. You can also link data sets across folders so that changes in one data set are reflected in another.
Choosing and Running the Appropriate Analysis
Prism 5 offers a wide range of analyses for different types of data and research questions. You can access the analysis dialog box by clicking Analyze on the toolbar or by right-clicking on a data table. Some of the analyses you can perform with Prism 5 are:
t tests, one-, two-, and three-way ANOVA, linear and nonlinear regression, dose-response curves, binary logistic regression, survival analysis, principal component analysis, and much more.
Each analysis has a checklist to help you understand the required statistical assumptions and confirm you have selected an appropriate test.
You can also customize various options for each analysis, such as confidence intervals, outliers detection, multiple comparisons corrections, residuals plots, etc.
Prism 5 will automatically generate a results table and a graph for each analysis. You can view these by clicking on the tabs at the bottom of the window.
Interpreting and Reporting Your Results
Prism 5 provides clear and comprehensive results for each analysis. You can view the results table by clicking on the Results tab at the bottom of the window. The results table will show you:
The summary statistics for each group or curve
The p-values and confidence intervals for each test or comparison
The parameter estimates and goodness-of-fit measures for each model
The post-hoc tests results for multiple comparisons
The survival curves and hazard ratios for survival analysis
The ROC curves and diagnostic accuracy measures for diagnostic tests
The principal components and loadings for principal component analysis
You can also view the results graph by clicking on the Graph tab at the bottom of the window. The results graph will show you:
The data points with error bars or symbols
The fitted curves with confidence aa16f39245